Thursday, 2 February 2012

Conducting the Blood Sample Collection Session

7. Conducting the Blood Sample Collection Session

7.1 Venipuncture

The type of equipment used for blood collection, and the post-collection process, will differ depending on the type of analysis required. The vaccutainers identified below are recommended as they have been fully validated by WADA and or WADA accredited laboratories. Alternate equipment which may meet the same criteria to those identified herein may be permissible but should be validated by WADA and/or the relevant laboratory, and consistent with the collection methodology presented herein, prior to use. In summary:

7.1.1 Collection of blood for analysis of Prohibited Substances and Methods in whole blood (e.g. detection of blood transfusion) or in plasma (e.g. HBOCs and CERA):

Number of Samples: 2 ("A" Sample and "B" Sample)

Volume required: 2 x 3mL (or as specified by relevant Laboratory)

(BD Vacutainer K2EDTA (K2) CE cat no 368856/ref US 367856) The tube used contains EDTA as anti-coagulant. The contents must be homogenized as soon as possible after collection. E.g. tubes should be gently inverted eight (8) to ten (10) times. The contents shall then be sent to Laboratory with no further action.

7.1.2 Collection of blood for analysis of
Prohibited Substances and Methods in serum (e.g. detection of hGH, HBOCs and CERA):

Number of Samples: 2 ("A" Sample and "B" Sample)

Volume required: 2 x 5mL (or as specified by relevant Laboratory) Blood is drawn into a tube that has an inert polymeric serum separator gel and a clotting activation factor (BD Vacutainer® SST II, EU ref 367955).

The contents must be homogenized as soon as possible after collection (e.g. tubes should be gently inverted up-side down at least five (5) times). The contents shall then be sent to Laboratory with no further action.


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